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| Preparation and Preliminary Characterization of Monoclonal Antibodies against E2 Protein of Bovine Viral Diarrhea Virus |
| WANG Shu1,2, ZHU Yuan-mao2, CAI Hong2, MA Lei2, SHI Hong-fei2, LÜ Chuang2, DONG Xiu-mei2, GAO Yu-ran2, XUE Fei2 |
1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150001, China;
2. Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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Abstract To prepare monoclonal antibody (MAb) against glycoprotein E2 of bovine viral diarrhea virus (BVDV), BALB/c mice were immunized with purified recombinant E2(rE2) expressed in E.coli and two hybridomas secreting MAb were screened from fusing the SP2/0 cells with the spleen cells of the immunized BALB/c mice by indirect ELISA coated with BVDV.The titers of MAb 4E3 and 1G11 in ascites were 6.21×106,6.83×105, 6.83×105, and 7.5×104 as detected by rE2 and BVDV, respectively. The MAbs secreted by hybridomas 4E3 and 1G11 had highly reactivity and specificity in indirect ELISA, Western blotting, IFA and identified as IgM with a light chain of κ by indirect ELISA.The specific tests indicated that the MAbs 4E3 and 1G11 had no reaction with bovine infectious rhinotracheitis virus,bovine parainfluenza virus type 3 and bovine adenovirus type 3.Meanwhile the MAb 4E3 had no reaction with classical swine fever virus(CSFV)and the MAb 1G11 had reaction with CSFV.Therefore the MAbs 4E3 and 1G11 might be used for differentiating BVDV and CSFV.On the other hand, the MAbs 4E3 and 1G11 could be used for establish diagnosis method for BVDV.
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Received: 03 December 2012
Published: 25 April 2013
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Cite this article:
WANG Shu, ZHU Yuan-mao, CAI Hong, MA Lei, SHI Hong-fei, LÜ Chuang, DONG Xiu-mei, GAO Yu-ran, XUE Fei. Preparation and Preliminary Characterization of Monoclonal Antibodies against E2 Protein of Bovine Viral Diarrhea Virus. China Biotechnology, 2013, 33(4): 40-45.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I4/40
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