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| Construction and Verification of an Plant Expression Vector pCAMBIA2300-35S-GUS-CaMVterm |
| GONG Yuan-yong1,2, Feng2, Yong-kun1, GUO Shu-qiao1, SHU Hong-mei1, NI Wan-chao2 |
1. Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Nanjing 210014, China;
2. Key Laboratory of Plant-Soil Interactions, Ministry of Education; Center for Resources, Environment and Food Security, China Agricultural University, Beijing 100193, China |
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Abstract Inorder to broaden the scope of use of plant expression vector pCAMBIA2300 and convenient to use for plant genetic engineering scientists in process of construction of expression vector, this research used SacⅠand XhoⅠdouble-digested pJIT166 vector obtaining 35S-GUS-CaMVterm fragment, then inserted this fragment to multiple cloning sites of pCAMBIA2300 vector, constructed the final expression vector pCAMBIA2300-35S-GUS-CaMVterm. The constructed expression vector was mediated by A. tumefacien (strain GV3101) using floral-dip method transformed to Arabidopsis thaliana. The GUS staining result of transgenic plants was further validated the correctness and practicality of this constructed vector. The construction of this expression vector was convenient for constructing gene over-expression vector,made it possible for studying promoter activity and GUS histochemical localization, and can also be used to identify transgenic plants. This constructed expression vector provided a better alternative plant expression vector for plant genetic engineering work.
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Received: 05 December 2012
Published: 25 March 2013
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