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| Mechanism of PTEN/NF-κB/Caspase Pathway on Adriamycin Resistance Reversal in K562/ADM Cells |
| CHENG Zhi-yong1, LIANG Wen-tong1, WANG Su-yun2, YAN Xiao-yan1, LI Hua1, WANG Bao-yan1, TIAN He1, WEI Yu-tao1, LU Xi 1 |
1. The No1 Hospital of Baoding, Baoding 071000, China;
2. Hebei General Hospital, Shijiazhuang 050051, China |
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Abstract Objective:To investigate the mechanism of PTEN/Akt/NF-κB signal pathway in drug resistance reversal on K562/ADM cells in vitro. Methods: The recombinated adenovirus containing green fluorescent protein and PTEN(Ad-PTEN-GFP)or empty vector (Ad-GFP) was transfected into K562/ADM cells together with different concentration of adriamycin(ADM). We detected the different drug IC50 with or without PTEN gene transfection to calculate the durg resistant factor (RF) by MTT; the proliferation of K562/ADM cells was observed by MTT assay; the apoptosis rate was assessed by flow cytometry (FCM). PTEN, NF-κB, I-κB, P53 mRNA were detected by real-time fluorescent relative-quantification reverse transcriptional PCR (FQ-PCR). PTEN, Akt, p-Akt and NF-κB protein levels were detected by western blot. Results: PTEN gene can enhance adriamycin sensitive in K562/ADM cells. The RF of ADM was 3.8 fold after transfection with PTEN gene. p-Akt and NF-Κb (P65) protein levels were down-regulated and NF-κB mRNA down-regulated too, but P53 up-regulated after transfaction with Ad-PTEN-GFP in K562/ADM cells, Caspase-3/7 activities up-regulated. PTEN mRNA expression levels were negative correlation with NF-κB mRNA(r=－0.968,P<0.05), NF-κB activities were negative correlation with Caspase-3/7 activities(r=－0.986,P<0.05). Conclusion: Wild PTEN gene might reverse adriamycin resistance via inhibiting PTEN/NF-κB/Caspase pathway.
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Received: 08 October 2012
Published: 25 March 2013
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Cite this article:
CHENG Zhi-yong, LIANG Wen-tong, WANG Su-yun, YAN Xiao-yan, LI Hua, WANG Bao-yan, TIAN He, WEI Yu-tao, LU Xi. Mechanism of PTEN/NF-κB/Caspase Pathway on Adriamycin Resistance Reversal in K562/ADM Cells. China Biotechnology, 2013, 33(3): 54-60.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I3/54
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