|
|
|
| Process Optimization of Selectively Enzymatic Synthesis of Isoquercitrin Using Ionic Liquid |
| SUN Guo-xia1, WANG Jun1, DING Wei-tong1, WANG Kai-xuan1, WU Fu-an 1,2 |
1. School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212018,China;
2. The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018,China |
|
|
|
Abstract To improve the yield of enzymatic synthesis of isoquercitrin, the process condition of enzymatic reaction was optimized by response surface methodology. According to the results of single-factors experiments, response surface methodology was investigated the selected three factors: ionic liquid content, enzyme concentration and substrate concentration. The result showed that the optimum condition for the enzymatic synthesis of isoquercitrin using ionic liquid as a co-solvent was: ionic liquid content 13.05%, enzyme concentration 9.10 g/L, substrate concentration 0.72 g/L. Under the optimal condition, the yield of isoquercitrin was 99.27 ? 0.55%, increased by 8.60% than before.
|
|
Received: 12 October 2012
Published: 25 March 2013
|
|
|
|
|
[1] 金越,吕勇,韩国柱,等. 槲皮素及异槲皮素、芦丁抗自由基活性的比较研究. 中草药,2007,38(3):408-412. Jin Y, Lv Y, Han G Z, et al. Comparative study on in vitro anti-free radical activities of quercetin, isoquercetin and rutin. Chinese Traditional and Herbal Drugs, 2007, 38(3): 408-412.
[2] Silva C G,Raulino R J,Cerqueira D M,et al. In vitro and in vivo determination of antioxidant activity and mode of action of isoquercitrin and Hyptis fasciculata. Phytomedicine, 2009,16(8): 761-767.
[3] Motoyama K,Koyama H,Moriwaki M,et al. Atheroprotective and plaque-stabilizing effects of enzymatically modified isoquercitrin in atherogenic apoE-deficient mice. Nutrition, 2009,25(4): 421-427.
[4] 张利斌,张晓庆,李玉平. 异槲皮苷抗抑郁作用实验研究. 药学实践杂志,2011,29(4):272-273. Zhang L B, Zhang X Q, Li Y P. Experimental study on the antidepressant activities of isoquercitrin. Journal of Pharmaceutical Practice. 2011, 29(4): 272-273.
[5] Shimada Y,Dewa Y,Ichimura R,et al. Antioxidant enzymatically modified isoquercitrin suppresses the development of liver preneoplastic lesions in rats induced by β-naphthoflavone. Toxicology, 2010, 268(3): 213-218.
[6] Salem J H,Humeau C,Chevalot I,et al. Effect of acyl donor chain length on isoquercitrin acylation and biological activities of corresponding esters. Process Biochemistry, 2010, 45(3): 382-389.
[7] Wang J,Zhao L L,Sun G X,et al. A comparison of acidic and enzymatic hydrolysis of rutin. African Journal of Biotechnology, 2011, 10(8): 1460-1466.
[8] Wang J,Ma Y L,Wu X Y,et al. Selective hydrolysis by commercially available hesperidinase for isoquercitrin production. Journal of Molecular Catalysis B: Enzymatic, 2012, 81: 37-42.
[9] Vidya P,Chadha A. Pseudomonas cepacia lipase catalyzed esterification and transesterification of 3-(furan-2-yl) propanoic acid/ethyl ester: A comparison in ionic liquids vs hexane. Journal of Molecular Catalysis B: Enzymatic, 2010, 65(1–4): 68-72.
[10] Lang M,Kamrat T,Nidetzky B. Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable β-glycosylhydrolase CelB from Pyrococcus Furiosus. Biotechnology and Bioengineering, 2006, 95(6): 1093-1100.
[11] Park S,Viklund F,Hult K,et al. Vacuum-driven lipase-catalysed direct condensation of l-ascorbic acid and fatty acids in ionic liquids: synthesis of a natural surface active antioxidant. Green Chemistry, 2003, 5(6): 715-719.
[12] Wang J,Sun G X,Yu L,et al. Enhancement of the selective enzymatic biotransformation of rutin to isoquercitrin using an ionic liquid as a co-solvent. Bioresource Technology. Doi: 10.1016/j.biortech.2012.10.098.
[13] Wang R,Chen P,Jia F,et al. Optimization of polysaccharides from Panax japonicus C.A. Meyer by RSM and its anti-oxidant activity. International Journal of Biological Macromolecules, 2012, 50(2): 331-336.
[14] 刘鹏,王泽南,张仕发,等. 丛梗孢酵母发酵产赤藓糖醇的响应面优化. 中国生物工程杂志,2011,31(5): 69-74. Liu P, Wang Z N, Zhang S F, et al. Optimization of erythriol production by Moniliella acetoabutans using response surface methodology. China Technology, 2011, 31(5): 69-74. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
