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| Effects of Sucrose Feeding Rate and pH Control on Nisin Production in Fermentor |
| WANG Rui1, DU Chao1, ZUO Fang-lei1, CHEN Shang-wu1,2 |
1. Department of Food Science and Engineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2. Academician Expert Workstation of Beijing Municipal Science and Technology Association, Polo Biology Science Park, Beijing 101113, China |
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Abstract Effects of the pH control and sucrose feeding rate during the fermentation period on the fermentation of Lactococcus lactis and the titer of nisin were mainly studied. Through the comparison with the nisin produced by Lactococcus lactis which not adjust the fermentation condition pH, it is found that titer of nisin yeild 1.2 times higher than that nisin without pH asjustment which adjust the fermentation condition pH to the optimum value of 6.8. The sucrose feeding rate was designed at 2 and 4 g/L·h, respectively, nisin titer increase about 50.5% by constant addition of sucrose at a feeding rate of 4 g/L·h.
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Received: 25 June 2013
Published: 25 December 2013
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