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| Application of Peptide Nucleic Acid in Molecular Biotechnology |
| WANG Jian-hua, GUO Ze-qin |
| Bioengineering College of Chongqing University, Chongqing 400044, China |
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Abstract Peptide nucleic acid (PNA) is a nucleic acid mimic in which the deoxyribose phosphate backbone has been replaced by a pseudo-peptide polymer to which the nucleobases are linked. PNA forms complexes with DNA following the Watson-Crick base-pairing rules. The PNA-DNA complexes exhibited high thermal stability and mismatch sensitivity. The high stability of these hybrids has been explained by the absence of negative charges along the PNA backbone. Furthermore, PNA is resistant to biological degradation and can bind complementary RNA or DNA sequences with extraordinary high affinity and specificity. PNA possesses many of the properties desired for a good antisense agent or antigene drugs, such as the detection of a single base mutate in nucleic acids, the diagnosis and detection by PCR molecular beacon, quantitative analysis of fluorescence in situ hybridization, genechip and biosensor technology and so on. Thus, the above-mentioned applications of PNA in molecular biotechnology in recent years and the prospect of its application were summarized.
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Received: 02 August 2012
Published: 25 January 2013
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