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| ε-Poly-L-lysine Production from Precursor L-lysine by Streptomyces sp. M-Z18 |
| CHEN Xu-sheng, REN Xi-dong, ZENG Xin, DONG Nan, MAO Zhong-gui |
| The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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Abstract To investigate the process of ε-poly-L-lysine (ε-PL) production from precursor L-lysine under different culture conditions of Streptomyces sp. M-Z18, it has developed conversion of precursor L-lysine for ε-PL production, combined with two-stage culture method, and with fermentation from glycerol, respectively. The results of experiment showed that two-stage culture method was used for conversion L-lysine to ε-PL and attained at 15 g/L with biotransformation of 3 g/L L-lysine; Furthermore, ε-PL production from glycerol fermentation coupled with L-lysine conversion achieved 33.76 g/L ε-PL and enhanced ε-PL productivity at 37.8%, compared with L-lysine-free fermentation. It was demonstrated that ε-PL production could be derived from precursor L-lysine, however, the efficiency of this conversion is needed further improved. It has indicated that the limit of ε-PL production is the biosynthesis of L-lysine in primary metabolism. Meanwhile, the results presented here provide a guidance for the ε-PL-producing strains primary metabolism improvement by metabolic engineering, and an efficient approach to enhancement of ε-PL production in scale fermentation.
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Received: 19 September 2012
Published: 25 January 2013
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