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Isolation and Identification of Liver Cancer Stem Cells and Analysis of Differentially Expressed MicroRNAs |
XU Ying-chen1, GUAN Li-dong1,2, ZHOU Jun-nian1, ZENG Quan1, YUAN Hong-feng1, LI Si-ting1, GUAN Zhao-xuan1, HE Li-juan1, NAN Xue1, CHEN Lin1, YUE Wen1, PEI Xue-tao1 |
1. Stem Cell and Regenerative Medicine Lab, Beijing Institute of Transfusion Medicine, Beijing 100850, China; 2. National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing 100050, China |
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Abstract The aim of the study is to isolate and identify liver cancer stem cells and screen the differentially expressed microRNAs. CD90+ cells were confirmed the existence in eight hepatocellular carcinoma cell lines and specimens by flow cytometry and immunofluorescence. Next, CD90+ cells isolated from MHCC97-H cell line by MACS. Drug chemoresistance assay showed CD90+ cells have a stronger drug resistance capacity than CD90- cells. CD90+ cells also have a stronger ability to initiate tumor formation than CD90- cells in a xenograft model in nude mice. Taken together, liver cancer stem cells may be enriched in CD90+ populations. Therefore, we examined the differentially expressed microRNAs between CD90+ cell and CD90- cells by means of microRNA chip. It is revealed that 92 microRNAs are differentially expressed between CD90+ cell and CD90- cells, of which 52 microRNAs are highly expressed in CD90+ cells and 40 microRNAs are highly expressed in CD90- cells.
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Received: 08 October 2012
Published: 25 January 2013
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Cite this article:
XU Ying-chen, GUAN Li-dong, ZHOU Jun-nian, ZENG Quan, YUAN Hong-feng, LI Si-ting, GUAN Zhao-xuan, HE Li-juan, NAN Xue, CHEN Lin, YUE Wen, PEI Xue-tao. Isolation and Identification of Liver Cancer Stem Cells and Analysis of Differentially Expressed MicroRNAs. China Biotechnology, 2013, 33(1): 1-7.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2013/V33/I1/1
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