| TECHNIQUES AND METHODS |
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| Comparison and Evaluation of Nine Methods in Detecting Listeria monocytogenes |
| LIU Ya-li1,2, LIU Fang3, HAN Shun-yu1, LIU Qing2, SUN Zhi-bo4, XIA Jun-fang1, ZHU Xiao-qing5 |
1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;
2. School of medical instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
3. Internation Travel Healthcare Center of Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730020, China;
4. School of Basic Medical Sciences, Lanzhou University, Lanzhou 730030, China;
5. Gansu Entry-Exit Inspection and Quarantine Bureau, Lanzhou 730020, China |
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Abstract Nine methods were used to detect Listeria monocytogenes in bacterial suspensions and artificial contaminated food extracts, including traditional biological methods, chromogenic medium method, TAS-ELISA, DNA Direct-PCR, Bacterium Direct-PCR, IC-PCR, Bio-PCR, SYBR Green Real time-PCR and TaqMan Real time-PCR, and the results were analyzed and compared. The result showed that Traditional biological methods had false positive. The lowest detectable limits of TAS-ELISA, DNA Direct-PCR, Bacterium Direct-PCR and IC-PCR were 106, 102, 105, 104CFU/ml. The detection sensitivity of chromogenic medium method, SYBR Green Real time-PCR was 102CFU/ml. Bio-PCR, TaqMan Real time-PCR had the highest sensitivity which could reach 101CFU/ml. Chromogenic medium and TAS-ELISA had the advantages of simple operation, and they were suitable for preliminary screening of large samples. IC-PCR was a sensitive, specific, rapid, economic technology, suitable for detecting micro-pathogens in large volume samples particularly. Bio-PCR and TaqMan Real time-PCR had high sensitivity and specificity, which adapted to recheck and research.
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Received: 13 February 2012
Published: 25 June 2012
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