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| Screening and Analysis of Genes Related to Xylose Fermentation to Ethanol in Candida tropiclis |
| XU Yong1,2,3, SHEN Chong1,2, QIU Xing-tian1,2, CAI Peng1,3, HUANG Min-ren3, YU Shi-yuan1,3 |
1. College of Chemical Engineering Nanjing Forestry University, Nanjing 210037, China;
2. Jiangsu Province Key laboratory of Green Biomass-based Fuel & Chemical, Nanjing 210037, China;
3. MEC Key Laboratory of Forest Genetics & Biotechnology, Nanjing 210037, China |
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Abstract The high quality forward and reverse SSH-cDNA libriries were constructed by suppressing subtractive hybridization (SSH) for a model xylose-metabolizing yeast strain Candida tropicalis. About 1 013 and 525 ESTs in two SSH-cDNA libriries were sequenced by Sequencer PRISM ABI3730. From the sequences data, 525 and 288 unigene were gotten and annotated and analyzed by GO classification, in addition 67 and 12 unigene were found as potential new genes. Some candidate genes were selected based on combination analysis method of the traditional metabolism theory and common genes deletion by unigene BLAST between two SSH-cDNA libriries, these genes were then tested and detected for relative quantitative comparison of gene transcript by RT-PCR technology. Some key genes related to sugar metabolism and ethanol fermentation were put forward, involving gene STP, HAGT, XR2, XDH1, XDH2 and ADH. It will benefit and provide theoretical guidance for related research on transcript profile, gene recombination, metabolic engineering and fermentation controlling of xylose metabolism and ethanol fermentation in Candida tropicalis and other xylose fermenting strains.
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Received: 15 June 2012
Published: 25 November 2012
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