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| Construction of Plant Expression Vector with Constitutive Activation DREB2A and Its Genetic Transformation to Tobacco |
| ZHAO Qing1,2, WANG Gang2, JI Jing2, JIN Chao2 |
1. School of Chemical Engineering and Technology, Tianjin 300072, China;
2. School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China |
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Abstract In order to investigate the function of DREB2ACA in plant salt-tolerance reaction and thus to explore its application value in genetic engineering. DREB2ACA was cloned using SOE-PCR, and was constructed in plant expression vector driven by rd29A promoter. Tobacco plants were genetically transformed with the vector. Integration of the T-DNA was confirmed using southern blot and expression of the target gene under salt stress were tested using RT-PCR. Compared to the control, transgenic lines had higher photosynthesis pigments, enhanced photosynthesis rate and lower MDA level under salt condition. All results demonstrate that DREB2ACA is probably a promising candidate gene for drought improvement in crops.
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Received: 25 July 2012
Published: 25 November 2012
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