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| Research Advances in Improvement of Xylitol Producing Strains by Genetic Engineering Technology |
| JIAO Jing-yu1,2, WU Mian-bin1,2,3, ZHAO Jiong-feng1,2, LIN Jian-ping1,2, YANG Li-rong1,2 |
1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China;
2. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
3. Zhejiang Key Laboratory of Antifungal Drugs, Zhejiang, Taizhou 318000, China |
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Abstract Xylitol as a five carbon sugar alcohol and low caloric content, has been widely used in medicine, food and chemical fields. Compared with chemical synthesis method, biological synthesis method has become a research focus because of its mild operation conditions, low energy consumption, and little environmental pollution. However, there also exist the leading obstacles to the commercial production of xylitol by bioconversion, such as the different xylose metabolic pathways existed in natural microorganisms, nonspecific nature of xylose reductase(XR), and requiring nicotinamide cofactors (NADH and NADPH) in biosynthesis of xylitol. Based on reviewing and analyzing the current understanding on biocatalytic routes to xylitol production, novel genetic engineering strategies through improving metabolic pathways of microorganisms which can improve the biological catalytic conversion of xylitol yield were emphasised.
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Received: 10 September 2012
Published: 25 November 2012
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