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| Progress in Improving the Supply of Precursors for Heterologous Expression of Polyketides |
| DUAN Yue-jiao, XUE Chao-you, LU Wen-yu |
| Department of Biological Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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Abstract Polyketides have been widely used in clinical because of their important biological activities. But in the natural strains there are various of limitations in the process of synthesis that affect the application of the polyketides. The heterologous expression can lift the restriction as a useful tool. The key limiting factor for heterologous expression of polyketides is the inadequate supply of intracellular precursors. An overview on strategies to improve the supply of the precursors in the typical heterologous expression host strains is provided: introduce the biosynthetic pathway of the precursors to the heterologous expression host bacteria to reconstruct the metabolic network, over-express the synthesis pathway of the heterologous precursor, inhibite metabolic branch flux, knockout within-derived metabolic pathways, to improve the supply of precursors. It provides a reference for research and industrial production of heterologous expression
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Received: 26 June 2012
Published: 25 November 2012
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