|
|
|
| Effect of Low Expression of CENP-E on Breast Cancer Cell Line MCF-7 |
| ZHAN Xiao-qin, HU Min, SONG Pei-pei, ZHANG Fan, LIU Chen, SHI Qiong, WENG Ya-guang |
| Key Laboratory for Clinical Laboratory Diagnostics of Education Ministry, College of Laboratory Medical, Chongqing Medical University, Chongqing 400016, China |
|
|
|
Abstract Objective To explore the role of mitotic protein CENP-E in cancer.Methods Plasmid vector containing CENP-E special short hair RNA(shRNA)was transfected into human breast cancer MCF-7 cells.Nest PCR and Western blot was used to detect CENP-E mRNA and protein level;the proliferation of cells with down-regulated CENP-E were analyzed by MTT assay;the apoptosis of cells were detected by flow cytometry(FCM);the migration and invasion ability of cells were evaluated by transwell assay;indirect immunofluorescence was used to detect CENP-E protein and cell mitosis.Results Compared with the control group,the CENP-E mRNA and protein level in MCF-7 cells transfected shRNA-CENP-E were decreased; the cell growth were inhibited in shRNA-CENP-E group(P<0.05);the results of FCM showed that the cell apoptosis ratio were obviously increased after down-regulated CENP-E;indirect immunofluorescence indicated CENP-E protein was reduced after transfected with shRNA-CENP-E,and the nucleus showed abnormal caryogram;but the cell ability of migration and invasion in shRNA-CENP-E group were increased(P<0.05). Conclusion: Down-regulated part of CENP-E gene expression in breast cancer MCF-7 cells can inhibit cell proliferation,induce cell apoptosis,meanwhile enhance the cell ability of migration and invasion.
|
|
Received: 19 July 2012
Published: 25 October 2012
|
|
|
|
|
[1] Weaver B A,Silk A D,Montagna C,et al.Aneuploidy acts both oncogenically and as a tumor suppressor.Cancer Cell, 2007,11: 25-36.
[2] Kops G J,Weaver B A,Cleveland D W.On the road to cancer: aneuploidy and the mitotic checkpoint.Nature,2005,5:773-785.
[3] Huszar D, Theoclitou M E, Skolnik J,et al. Kinesin motor proteins as targets for cancer therapy. Cancer Metastasis Rev,2009,28:197-208.
[4] Janssen A,Medema R H.Mitosis as an anti-cancer target.Oncogene,2011, 30:2799-2809.
[5] Weaver B A, Cleveland D W.Does aneuploidy cause cancer.Current Opinion in Cell Biology 2006, 18:658-667.
[6] Janssen A,Kops G J,Medema R H.Targeting the mitotic checkpoint to kill tumor cells.Horm Canc,2011, 2:113-116.
[7] Yao X B,Abrieu A,Zheng Y,et al.CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint.Nature Cell Biology,2000, 2:484-491.
[8] Tanudji M, Shoemaker J, Lawrence L I,et al.Gene silencing of CENP-E by small Interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay.Molecular Biology of the Cell,2004, 15:3771-3781.
[9] Weaver B A, Bonday A Q,Putkey F R,et al.Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss.The Journal of Cell Biology, 2003,162(4): 551-563.
[10] Wood K W,Chua P, Sutton D,et al.Centromere-associated protein E: A motor that puts the brakes on the mitotic checkpoint.Clin Cancer Res,2008,14(23): 7588-7592.
[11] 刘子杰,翁亚光,李素彦等.用FRET方法研究与Mps1蛋白有相互作用的CENP-E蛋白结构域. 中国生物工程杂志,2009,29(4):28-34. Liu Z J, Weng Y G,Li S Y,et al. Study on the interaction between Mps1 and the structural domains of CENP-E protein by FRET. China Biotechnology, 2009,29(4):28-34.
[12] Mao Y,Abrieu A,Cleveland D W.Activating and silencing the mitotic checkpoint through CENP-E-dependent activation/inactivation of BubR1.Cell,2003,114(1): 87-98.
[13] 闫琛,凌康,翁亚光.CENP-E变异体在不同肿瘤细胞株及癌组织中的差异表达.中国生物制品学杂志.2010,23(7):678-682. Yan C,Ling K,Weng Y G.The expression difference and function of two CENP-E mRNA forms in different tumor tissues.Chin J Biologicals, 2010,23(7):678-682.
[14] Janssen A,Kops G J,Medema R H.Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells. PNAS,2009,106(45): 19108-19113.
[15] Kops G J,Foltz D R,Cleveland D W.Lethality to human cancer cells through massive chromosome loss by inhibition of the mitotic checkpoint.PNAS, 2004, 101(23):8699-8704. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
