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| Effects of Different Nitrogen and Carbon Sources on the Production of Antifungal Lipopeptides from Bacillus amyloliquefaciens Q-426 |
| ZHAO Peng-chao1,2, QUAN Chun-shan3,4, JIN Li-ming3,4, WANG Li-na1,2, FAN Shen-di 3,4 |
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China;
3. Department of Life Science, Dalian Nationalities University, Dalian 116600, China;
4. SEAC-ME Key Laboratory, Dalian 116600, China |
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Abstract Cyclic lipopeptides, which are produced by several types of microorganisms, will have great potential in the agricultural and biomedical field with their unusual antibacterial, antifungal and antiviral activities. To provide a theoretical basis for investigating the gene regulation of antifungal lipopeptides and enhancing their yield more effectively in the next step, effects of three factors (nitrogen sources, carbon sources and initial pH in the medium) on the production of bacillomycin D and fengycins from Bacillus amyloliquefaciens Q-426 were analyzed by measurement of antifungal activities against Curvularia lunata (Walk) Boed, reverse-phase high performance liquid chromatography (RP-HPLC), HPLC-mass spectrometry (MS) and MS/MS. Finally, the results were screened as follows: L-histine, L-lysine, glycerol, sorbitol and [OH]- in the medium could promote the accumulation of bacillomycin D, but less effective to that of fengycins. However, further analysis of RP-HPLC spectrum revealed the three factors above played different roles in the pathway of bacillomycin D biosynthesis from strain Q-426.
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Received: 18 June 2012
Published: 25 October 2012
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