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| Abiotic Stress Tolerance Analysis Two Alternatively Spliced Isoforms of LEA3 Gene from Pogonatherum paniceum in Yeast |
| LI Rui1, WANG Wen-guo2, FAN Lin-hong1, WANG Sheng-hua 1 |
1. College of Life Sciences, Sichuan University, Department of Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu 610064, China;
2. Research Centre of Biomass Energy Technology, Biogas Institute of Ministry of Agriculture,Chengdu 610041, China |
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Abstract It was to analyze the sequence of Pogonatherum paniceum group 3 LEA proteins alternatively spliced isoforms, and to detect the abiotic stresses tolerance of PpLEA3 spliced isoforms. The two spliced isoforms of PpLEA3 gene were amplified by PCR reaction using the plasmids pMD19-T- PpLEA3.a and pMD19-T- PpLEA3.b as the templates, respectively. The yeast expression plasmid of pYES2- PpLEA3.a and pYES2- PpLEA3.b was constructed and then transformed into yeast to create recombinant INV- PpLEA3.a and INV- PpLEA3.b . Stress tolerance tests showed that LEA3 yeast transformants exhibited a higher survival rates than the control transformants under salt (NaCl), NaHCO3, freezing, drought and ultraviolet radiation. PpLEA3.a has stronger abiotic stresses tolerance than PpLEA3.b . The nucleic acid sequence of two splicing isoforms have different protein hydrophilicity and structure which leading to differences in the stress tolerance.
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Received: 13 March 2012
Published: 25 August 2012
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