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| Selection of scFv Genes of Foot-and-Mouth Disease Virus Using Ribosome Display |
| DAI Peng, ZHOU Guang-qing, LIN Mi, FENG Xia, MA Jun-wu, ZHANG Xiao-xia |
| State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Gansu Provincial Engineering and Technique Research Centre on Biological Detection, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China |
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Abstract Objective: To screen the specific single-chain variable Fragment (scFv) gene of Foot-and-Mouth Disease by Ribosome display. Methods: Based on the construction of ribosome display library, five rounds of in vitro transcription, in vitro translation, affinity selection and RT-PCR constructed by ribosome display technology, then sequencing and analysis. Results: ScFv genes of FMDV were screened, and were enriched. Conclusion: Targeted by antigen of FMDV and purified 146S virus particle, FMDV scFv genes were obtained by using ribosome display technology. Not only previous basis for basic research, immunology research, prevention, treatment and diagnostic of FMD, but also for the development of rapid diagnostic techniques of FMD by using single-chain variable fragment were provided.
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Received: 21 March 2012
Published: 25 July 2012
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