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| Effect of GALNT14 on the Migration of Human Breast Cancer Cells MCF-7 |
| WU Chen, TIAN Huan-na, WANG Yuan-yuan, LIU Fang-ming, ZHANG Xiao-kang, LI Qin-jian, XIE Yuan-yuan |
| Life Science College, Hebei University, Baoding 071002, China |
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Abstract The recombinant plasmid—pcDNA 3.1-Flag-T14 was constructed and identified by restriction enzyme digestion and DNA sequencing. pcDNA 3.1-Flag-T14 was transfected into MCF-7 cells with lipofectamine 2000, and positive clones were selected by G418. The expression of GALNT14 in MCF-7 was tested by RT-PCR and Western blot. Wound healing assay and transwell migration assay were performed to detect migration activity. The effect of GALNT14 on the expression of MMP-2,MMP-9,TGF-β1and VEGF was measured by semi-quantitative RT-PCR. The result showed that pcDNA 3.1-Flag-T14 was successfully constructed and GALNT14 was expressed stably in MCF-7 cells. GALNT14 can promote the migration of MCF-7 cells. RT-PCR showed that GALNT14 also increased the expressions of MMP-2,MMP-9,TGF-β1and VEGF mRNA. In conclusion,GALNT14 can significantly improve the migration of MCF-7 cells and it might play an important role in the invasion and metastasis of tumor.
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Received: 06 February 2012
Published: 25 July 2012
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