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| Effect of Interfacial Ligands on the Absorption Behavior of BSA |
| MA Hong-mei1,2, ZHANG Gui-feng2, LI Chun1,3, KONG Ying-jun2, GAO Fei2, HU Tao2, MA Guang-hui2, SU Zhi-guo2 |
1. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;
2. National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
3. School of Life Sciences, Beijing Institute of Technology, Beijing 100081, China |
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Abstract The effect of interfacial ligands on the adsorption behavior of BSA was investigated using dual polarization interferometry (DPI). The sensor chips of DPI were modified with 3-aminopropyltriethoxysilane (APTES), (3-methylaminopropyl)trimethoxysilane (MAPTMS) and (3-diethylaminopropyl)trimethoxysilane (DAPTMS) respectively. The ligand densities on the modified chips were compared using X-ray photoelectron spectroscopy. The adsorption behaviors of BSA on the three modified chips were characterized using DPI and atomic force microscopy (AFM) respectively. It was found that the adsorbed BSA on the APTES-modified chip occupies a larger area than other ligand-modified ones, suggesting that the higher ligand density led to the more points in binding sites. The ligand density on MAPTMS-modified chip was similar as that on the DAPTMS-modified chip. BSA showed higher BSA adsorption on the DAPTMS-modified chip than the MAPTMS-modified, but the thickness of BSA layers were almost the same, indicating a higher density of BSA adsorbed. AFM images of the BSA adsorbed on the three ligand-modified chips corresponded to the result from DPI detection. The results indicates that the ligand density on the modified chip affects the amount, density and aggregation of BSA on the interfaces.
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Received: 28 February 2012
Published: 25 July 2012
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