Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells

China Biotechnology

2012, Vol. 32

Issue (07): 26-30 DOI:

Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells

WANG Xiao-xia^1,2, LU Na¹, LIU Zhi-rong¹, XIE Jun¹, WANG Hui-zhen¹, NIU Bo¹, PEI Yi²

1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China;
2. Shanxi Academy of Medical Sciences, Shanxi Dayi Hospital, Taiyuan 030032, China

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Abstract Objective:To establish Aurora-A overexpressing transfectant clones and investigate its effect on invasiveness as well as protein expression and activity of MMP-9 in esophageal carcinoma cells. Methods:The Aurora-A expressing plasmid was transfected using Lipofectamine^TM 2000 and the identification of transfected clones was confirmed by Western blot analysis and RT-PCR. The invasive properties of cells were examined by an in vitro Boyden chamber invasion assay. Level of MMP-9 protein expression and activity were measured with Western blot and ELISA as well as gelatin zymography analysis. Results:Aurora-A overexpressing transfectant clone was established, which increased cell invasion as well as protein expression and activity of MMP-9. Conclusion:The elucidation the molecular mechanism for the promotion of invasion in esophageal carcinoma by Aurora-A may lead to the development of new target for effective therapy.

Key words： Aurora-A Invasion MMP-9

Received: 31 January 2012 Published: 25 July 2012

ZTFLH:

R734.2

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	WANG Xiao-xia
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Cite this article:

WANG Xiao-xia, LU Na, LIU Zhi-rong, XIE Jun, WANG Hui-zhen, NIU Bo, PEI Yi. Effect of Aurora-A Overexpression on Invasion and MMP-9 Expression of Esophageal Carcinoma Cells. China Biotechnology, 2012, 32(07): 26-30.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2012/V32/I07/26

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