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| Cloning and Transient Expression Analysis of PtLFY Promoter from Populus tomentosa |
| LI Hao, CHEN Zhong, LI Ying, WANG Jia, AN Xin-min |
| National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing Forestry University, Beijing 100083, China |
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Abstract In order to study the expression and regulation of LEAFY homolog in Populus tomentosa, a 1575 bp 5' flanking sequence of PtLFY gene was cloned by PCR from genomic DNA of Populus tomentosa. The results analyzed by PLACE and PlantCARE showed that the sequence contains TATA-BOX,CAAT-BOX and other regulatory elements, such as MYB binding site involved in drought-inducibility, cis-acting regulatory element involved in the ABA responsiveness, light-responsive elements and other transcription factor-binding sites. It was supposed that the expression of PtLFY was regulated by drought, ABA and light etc. Compared the PtLFY promoter with that of other 5 species by FootPrinter, Both conservation and diversity were found in these promoter sequences. It indicated LEAFY homologues genes perform a similar function in Plants. Furthermore, a plant expression vector carrying PtLFYp∷GUS, called PtLFYp1304, was constructed. The results of Agrobacterium-mediated transient expression showed that the PtLFY promoter was competent to drive GUS gene expression in roots, stems, leaves and floral organs of Nicotiana tobaccum. Compared with CaMV 35S promoter, only slight expression of GUS gene was detected in roots, stems and leaves, whereas strong GUS expression driven by PtLFY promoter in the sepal and stamen of Nicotiana tobaccum.
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Received: 24 October 2011
Published: 25 April 2012
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