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Compare the Effect of NRP-1 Antisense Oligodeoxynucleotides With That of VEGFR-2 Antisense Oligodeoxynucleotides on the Proliferation and Apoptosis of Human Gastric Cancer SGC7901 Cells |
LI Ben-jie, WU Xiao-ling, WANG Lu-lu |
Department of Gastrointestinalogy, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China |
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Abstract Objective: To investigate and compare the effect of neuropilin-1(NRP-1) antisense oligodeoxynucleotides with that of vascular endothelial growth factor receptor-2 (VEGFR-2) antisense oligodeoxynucleotides on the proliferation and apoptosis of human gastric cancer SGC7901 cells. Methods: Transmit thiophosphorylate modified antisense oligodeoxynucleotides of NRP-1 and VEGFR-2 into human gastric cancer SGC7901 cells with liposome separately and simultaneously, detect NRP-1 mRNA levels and VEGFR-2 mRNA levels by reverse transcriptase-polymerase chain reaction(RT-PCR); Measure the changes of cell proliferation by methyl thiazolyl tetrazolium(MTT); Observe cell apoptosis by flow cytometry. Results: The transcription level of NRP-1 mRNA and VEGFR-2 mRNA in SGC7901 cells after transmission with ASODN decreased. NRP-1 ASODN and VEGFR-2 ASODN inhibit the proliferation of SGC7901 cells and meanwhile promotes the apoptosis of these cells. Such effect intensifies as the concentration of ASODN increases; When NRP-1 ASODN and VEGFR-2 ASODN are used separately, no significant difference is detected. Conclusion: NRP-1 ASODN and VEGFR-2 ASODN inhibited the transcription level as well as the proliferation while they promote the apoptosis level of SGC7901 cells. Compared with that of transmitting ASODNs separately,the effect manifests itself more significantly with the combination of NRP-1 ASODN and VEGFR-2 ASODN.
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Received: 30 November 2011
Published: 25 April 2012
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