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| Effects and Possible Mechanism of BMP9 on the Bone Metastasis of Human Breast Cancer Cells MDA-MB-231 |
| SUN Xiao-xiao1, WANG Ke2, FENG Hong-lei1, LIU Yue-hong1, WAN Shao-heng1, LUO Jin-yong1, ZHANG Yan1 |
1. The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education, Chongqing Medical University, Chongqing 400016, China;
2. The Laboratory Department, Affiliated Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China |
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Abstract The purpose is to investigate the effects and possible mechanisms on BMP9 inhibiting the bone metastasis of human breast cancer MDA-MB-231 cells. High titer adenovirus vector expressing BMP9 was used to infect MDA-MB-231 cells. Experimental group is MDA-MB-231/BMP9 cells,control groups are both MDA-MB-231/GFP cells and MDA-MB-231 cells. RT-PCR and Western blot were used to detect the expression of BMP9 and PSmad1 in recombinant MDA-MB-231/BMP9 cells; Q-PCR and Western blot were used to detect the expression of CTGF in three groups cells; At last, X Light and Immunohistochemistry were used separately to detect the osteolytic lesions and the expression of CTGF in three groups cells. BMP9 was expressed in recombinant MDA-MB-231/BMP9 cells; the recombinant MDA-MB-231/BMP9 cells exhibited higher level of phosphorylated Smad1 and lower level of CTGF than the MDA-MB-231/GFP cells and MDA-MB-231 cells, but total Smad1 protein was similar in the three groups. In comparison with the control groups, the osteolytic lesions observed were significantly reduced in MDA-MB-231/BMP9 group; The removed tumor samples were detected by immunohistochemistry, MDA-MB-231/BMP9 samples analysed showed less CTGF staining than the control samples. Taken together, BMP9 can inhibit the bone metastasis of breast cancer cells MDA-MB-231 in vivo and the downregulation of CTGF is the possible mechanism.
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Received: 12 December 2011
Published: 25 March 2012
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