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| Effect of the Incubation Time in the Enrichment of the Phosphopeptides with Titanium Dioxide |
| ZHAN Sheng, YIN Xing-feng, LI Hui, LI Nan, YANG Xiao-yan, SUN Xue-song |
| Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China |
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Abstract Objective: Aims to explore the effect of the incubation time in the enrichment of the phosphopeptides with Titanium dioxide. Methods: Firstly, the optimum ratio between beads and peptides based on the preliminary study is ascertained, and then compare the effect of the incubation time on the enrichment with Titanium dioxide. Results: The optimal ratio between bead and peptide is 3 :1. Under this optimized ratio, the longer incubation time decreases bead selectivity and 5 minutes is the optimal incubation time. Conclusion: The incubation time has the negative effect on the phopshopeptide enrichment which indicates that the optimal time depends on sample species.
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Received: 20 October 2011
Published: 25 March 2012
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