|
|
|
| The Research of the Highly Effective Biocontrol Emulsion with Bacillus subtilis |
| LIU Jin-xia, Ll Na, DU Wen-jing, WU Jian-rong, LI Jin, DING Pin, SHEN Si-yuan, ZHANG Jian-jun |
| Institute of Biology, Gansu Academy of Sciences,Lanzhou 730000, China |
|
|
|
Abstract Taking co-fermentation both of Bacillus subtilis D17 and Bs16 (1:1) as based drugs, through the compound design, the preparation stability, resistant, the indoor outside antibacterial bioactivities experiment, carrying out best emulsion screening. The result shows that: the toxicity of emulsion 1 and emulsion 9 was the strongest. It has the high control efficiency to the grey mold. It may also reach above 85% in the plot experiment to the grey mold's against effect. Its control efficiency is similar with the common chemistry agricultural chemicals. Its effective date which lengthens to 15 days above has the significance difference with the common chemistry agricultural chemicals. Its anti-ultraviolet ability, passed through 180 minute ultraviolet illumination preparation unit volume the effective live fungus quantity still to be possible to reach above 109cfu/ml, is very strong. The storage stability is good, the normal temperature seal evades under the cloudy condition to guarantee the nature time to be possible to amount to above one year. It would be the new green environmental protection biology agricultural chemicals which has the high production and the commodity development value.
|
|
Received: 14 March 2011
Published: 25 September 2011
|
|
|
|
|
|
[1] 周燚,王中康,喻子牛. 微生物农药研发与应用.北京:化学工业出版社,2006:25-53. Zhou Y, Wang Z K,Yu Z N. Microbial Pesticide Research Development And Application. Beijing: Chemical Industry Press, 2006:25-53.
[2] Arras G. Inhibition of postharvest fungal pathogens byBacillus subtilisstrains isolated from citrus fruit. Advances in Horticul-tural Science, 1993, 7(3):123-127.
[3] 刘雪,穆常青,蒋细良,等.枯草芽孢杆菌代谢物质的研究进展及其在植病生防中的应用. 中国生物防治,2006,22:179-184. Liu X, Mu C Q, Jiang X L, et al. Chinese Journal of Biological Control, 2006, 22: 179-184.
[4] Office of Pesticide Program. Regulating Biopesticides. . http://www.epa.gov/pesticides/biopesticides.
[5] 朱昌雄,丁振华,蒋细良,等.微生物农药剂型研究发展趋势. 现代化工, 2003,23(3):4-8. Zhu C X, Ding Z H, Jiang X L, et al. Modern Chemical Industry,2003, 23(3): 4-8.
[6] 赵军.廉价环保型农药乳剂技术的研究.济南:山东大学,2007. Zhao J. Inexpensive Environmental Protection Agricultural Chemicals Emulsion Technology Research. Jinan: Shandong University, 2007.
[7] 许时婴,张晓鸣,夏书芹,等.微胶囊技术——原理与应用.北京:化学工业出版社,2006,20-30. Xu S Y, Zhang X M, Xia S Q, et al. Microcapsule Technology and Application. Beijing: Chemical Industry Press, 2006, 20-30.
[8] 孙广宇,宗兆锋.植物病理学实验技术.北京:中国农业出版社,2002:143-145. Sun G Y, Zong Z F. Experimental Technique of Plant Pathology. Beijing: China Agriculture Press, 2002:143-145.
[9] 农业部农药检定所生测室主编.农药田间药效试验准则.北京:中国标准出版社,2000:190-193. The measurement chamber of the ministry of agriculture pesticide testing institute. Pesticide Guidelines for the Field Efficacy Trids. Beijing: Standards Press of China, 2000:190-193.
[10] 中国农业百科全书总编辑委员会.中国农业百科全书(植物病理学卷).北京:农业出版社,1996. Chinese Agriculture Encyclopedia Editor-in-chief Committee. Chinese Agriculture Encyclopedia (Volume of Plant pathology). Beijing: Agriculture Press, 1996.
[11] 胡林,徐汉虹,梁明龙.鱼藤酮水基纳米浮剂的特性及对松材线虫的杀灭作用. 农药学学报,2005, 2:171. Hu L, Xu H H, Liang M L. Chinese Journal of Pesticide Science, 2005, 2:171.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
