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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (9): 62-68    DOI:
    
Optimization of Mixed Fermentation on Cottonseed Meal and Research on the Synergistic Effect of B.subtilis H1 and A.niger P1
ZHUGE Bin1, LIU Jun1, FANG Hui-ying1, SUN Jin2, ZHANG Meng3, ZHUGE Jian1
1. The Key Laboratory of Industrial Biotechnology, Ministry of Education and the Research Centre of Industrial Microbiology, Jiangnan University, Wuxi 214122,China;
2. Zhejiang Zhengwei Food Co. Ltd., Yiwu 322002,China;
3. Department of Biotechnology, Durban University of Technology, Durban 4000, South Africa
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Abstract  

Study on the optimization of B.subtilis H1 and A.niger P1 mixing fermentation to degrade the macromolecular protein of cottonseed meal was carried out. The results showed that the optimal conditions were as follows: cottonseed meal 40 g, (NH4)2SO4 0.2%, bran 15%, water content 50%, nature pH 6.0, temperature 30 ℃, the optimal time 60 h, the optimal inoculation concentration was 15% and the optimal inoculation ratio 2 ∶ 1 (A.niger P1 ∶ B.subtilis H1). Under the optimal level determined, the content of small peptide increased to 18.36%, the length of average peptide chain decreased to 4.23 and the digestibility of protein increased to 88.59%.A.niger P1 can produce rich acid protease, while B.subtilis H1 can produce rich neutral and alkali protease. Under the combined fermentation, the enzyme activity significantly increased. The peptides composed of more than 10 amino acids in the water-soluble peptides were almost degraded to peptides composed of 1~3 amino acids. In short, combined fermentation was better than single fermentation. The two strains have synergistic effect.

Key words： Cottonseed meal      B.subtilis      A.niger      Combined fermentation      Small peptide     
Received: 06 April 2011      Published: 25 September 2011
ZTFLH:  Q786  
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Cite this article:

ZHUGE Bin, LIU Jun, FANG Hui-ying, SUN Jin, ZHANG Meng, ZHUGE Jian. Optimization of Mixed Fermentation on Cottonseed Meal and Research on the Synergistic Effect of B.subtilis H1 and A.niger P1. China Biotechnology, 2011, 31(9): 62-68.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I9/62


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