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| Lentiviral Vector Mediated GHRH Gene Expression in Mouse Muscle and the Effect on Growth of Animals |
| DAI Jian-wei1,3, ZHANG Qian-qian4, LIU Song-cai3, ZHANG Ming-jun3, ZHANG Yong-liang2 |
1. Department of Biochemistry, Guangzhou Medical College, Guangzhou 510182, China;
2. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
3. Department of Biochemistry, Veterinary College, Jinlin University, Changchun 130062, China;
4. Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-Sen University, Guangzhou 510275, China |
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Abstract Overexpression of Growth hormone-releasing hormone (GHRH) can control the synthesis and secretion of the growth hormone (GH) from the hypophysis as well as regulate the GH concentrations in animals and humans. The lentiviral vector system were modified to be a CMV-SP two promoter vector. GHRH cDNAs were cloned into the vector and transfected 293T cells with other three plasmids to package LV-SP-GHRH pseudovirion. Muscle tissue of mice were injected with the virus particles and the animal weight growth were observed. At the same time viral safety was assessed by detection of whether the virus structural genes integrated into the genome of infected cells. The results showed that the body weight gain per day increased 153.85% compared to the control group at 28th day postinjection, and the genome of infected cells without virus structural gene integration. All the results indicated that lentiviral vector mediated GHRH gene can promote animal growth significantly and safely.
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Received: 05 January 2011
Published: 25 July 2011
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