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| Surface Chemistry Modulates Fibronectin Conformation and Control Cell Behavior |
| HUO Run-lan, LUO Yan-feng, LI Yan, YAN Wei-wei, XING Juan, LIN Fu-chun, WANG Yuan-liang |
| Key Laboratory of Biorheological Science and Technology, Bioenginering College of Chongqing University, Research Center of Bioinspired Material Science and Engineering, Chongqing 400030, China |
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Abstract Biomaterial surface properties modulate protein adsorption and cell adhesion, proliferation, spreading to elicit diverse cellular responses in biomedical and biotechnological applications. Alkanethiol self-assembled monolayers (SAMs) presenting well-defined chemistries (NH2, COOH, OH and CH3) were employed to analyze the effects of surface chemistry on controlling fibronectin (FN) conformation as well as osteoblasts behaviors. X-ray photoelectron spectroscopy (XPS) is used to confirm the presence of well-formed monolayers of expected physicochemical characteristics, and the hydrophilicity of SAMs was characterized by water contact angle. Immunostaining were applied to indicate conformation of FN. In addition, the effects of SAMs with pre-coated FN on the cytoskeleton organization and proliferation were also examined. Water contact angle data reveals the-CH3groups lead to a hydrophobic surface, while COOH, OH groups lead to a hydrophilic surface and NH2groups a neutral surface. SAMs presenting terminal NH2, COOH, OH and CH3 functionalities modulated adsorbed FN conformation as determined through differences in the binding affinities of monoclonal antibodies raised against the central cell-binding domain,-OH surfaces exhibited a statistically greater HFN 7.1 positive area than the other surfaces. Then, the changes in osteoblasts proliferation and spreading correlated with the FN conformation.
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Received: 04 March 2011
Published: 25 July 2011
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