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| Optimization of Erythritol Production by Moniliella acetoabutans Using Response Surface Methodology |
| LIU Peng, WANG Ze-nan, ZHANG Shi-fa, LI Ying |
| School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China |
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Abstract The culture medium and fermentation conditions of erythritol production by Moniliella acetoabutans was optimized by response surface methodology. According to the results of mono-factors experiments, a Plackett-Burman design was used to investigate and evaluate the influence of related factors, three factors are statistically significant: glucose concentration, initial pH value and temperature. Box-Behnken was employed to design. The quadratic regression analysis was conducted on the optimized results by using SAS software, it was found that the optimum conditions for erythritol production were glucose26%, yeast extract 0.4%, KH2PO4 0.2g/L,MnSO4·4H2O 0.05g/L,CuSO4·5H2O 0.04g/L, initial pH value 4.2, temperature 31℃, broth content 20%, shaking speed 150r/min, inoculum 4%. Under this condition, the concentration of erythritol reached to 75.63g/L in the shake-flask experiments after 90h fermentation, increased by 10.8% than before, the yield was 36.12%, increased by 9.4% than before.
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Received: 28 December 2010
Published: 27 May 2011
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