Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library

China Biotechnology

2011, Vol. 31

Issue (5): 104-107 DOI:

Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library

WANG Zi-ye, FENG Juan, MA Xue-mei, WANG Ming-lian, ZHONG Ru-gang

College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China

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Abstract

A phage display peptide library was screened by targeting recombinant p24 antigen. After three rounds of panning, eleven phage clones were chosen to test the binding affinity by ELISA, and ten of them were positive. DNA sequencing showed that nine of the positive clones display a unique sequence FTTRANA which has high affinity with HIV-1 p24 antigen. To improve the condition of phage ELISA, phage number and blocking buffer have been optimized, phage number ranged from 8.13×10¹¹ to 1.02×10¹⁴ pfu/well and 0.5%BSA was highly recommended. Therefore, in this study, peptides combined with p24 antigen were obtained which might be useful in p24 antigen assay.

Key words： Phage display Peptide HIV-1 p24

Received: 20 December 2010 Published: 27 May 2011

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WANG Zi-ye, FENG Juan, MA Xue-mei, WANG Ming-lian, ZHONG Ru-gang. Screening of HIV-1 p24 Binding Peptides from Phage Display Peptide Library. China Biotechnology, 2011, 31(5): 104-107.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I5/104

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