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| Production and Characterization of Anti-OAS1 Monoclonal Antibody |
| LI Zi1, BAO Xin-chen1, GAO Xiang-dong1, LU Xiao-dong2, XU Chen2 |
1. College of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;
2. Beijing Tri-Prime Genetic Engineering Incorporated, Beijing 102600, China |
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Abstract Aim: To detect OAS1, its monoclonal antibody was prepared by immune mouse. Methods: BALB/c female mice were immunized with His-OAS1 expressed by E.coli BL21 cells. Monoclonal antibody (McAb) was prepared by hybridoma technology. Identify the purity and characteristics of the selected McAb purified with saturated ammonium sulfate and give preliminary evaluation of the effectiveness of its application, in which SDS-PAGE, ELISA, Western blot and other methods were used. Results: High efficient expression of OAS1 protein in vivo, a McAb can detect the OAS1 was obtained designated as C24-5. The titer of C24-5-McAb was over 9×10-10mol/L, and the affinity constant of C24-5-McAb was 7.66×107 L·mol-1. Conclusion: The preparation of C24-5-McAb is very important to further improve the detection of OAS1.
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Received: 03 May 2011
Published: 25 October 2011
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