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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (10): 63-67    DOI:
    
Escherichia coli Expression, Purification and Functional Identification of Farnesol Synthase from Artemisia annua L.
LI Zhen-qiu, JIN Ya-ming, WANG Bo
College of life Sciences, HeBei University, Baoding 071002, China
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Abstract  

The open reading frame of sesquiterpene cyclase cDNA (AF304444) from Artemisia annua was subcloned into a bacterial expression vector pET30a(+) in frame with N-terminal and C-terminal HIS6-tag, then recombinant vector pET30SESQ was introduced into Escherichia coli strain BL21(DE3). Recombinant sesquiterpene cyclase was induced at 28℃ by adding 0.5 mmol/L IPTG (Isopropyl-beta-D-thiogalactoside) and purified using immobilized metal affinity chromatography on Ni2+ columns. GC-MS analysis showed that the recombinant sesquiterpene cyclase can catalyze the formation of farnesol from FPP.



Key wordsFarnesol synthase      Escherichia coli expression      Functional identification      Artemisia annua L.     
Received: 16 March 2011      Published: 25 October 2011
ZTFLH:  Q786  
Cite this article:

LI Zhen-qiu, JIN Ya-ming, WANG Bo. Escherichia coli Expression, Purification and Functional Identification of Farnesol Synthase from Artemisia annua L.. China Biotechnology, 2011, 31(10): 63-67.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I10/63


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