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| Effects of Nitrogen-containing Media on the Accumulation of Lipid and Carbohydrate in Marine Microalgae Isochrysis zhanjiangensis |
| FENG Di-na1,2, AI Jiang-ning1, LIU Ya-nan1, CHEN Zhao-an1, XUE Song1, ZHANG Wei3,4 |
1. Marine Bioproducts Engineering Group, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
3. Flinders Centre for Marine Bioprocessing and Bioproducts (FCMB2) Flinders University, Aldelaide SA5042, Australia;
4. Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide SA5042, Australia |
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Abstract Effects of eight media on the growth, PSⅡ activity, lipids and carbohydrate accumulation of a marine microalgae Isochrysis zhanjiangensis were investigated. When the nitrogen concentration in the medium is 1.5 g/L, the highest lipid content of 39.8 % and the maximum lipid yield of 0.92 g/L were achieved, while the carbohydrate content of 11.6 % was the lowest. When the nitrogen concentration in the medium is 0.016 g/L, the lowest biomass of 0.74 g/L and the lowest lipid content of 21.1 % were obtained. However, the highest carbohydrate content of 44.4 % was obtained. Based on the result of linear fitting equation, the concentration of NO3-in the medium has a good positive correlation with total lipid content of algal cells. Therefore, the regulation in the yield of lipids and carbohydrate is realized by studying different nitrogen-level media.
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Received: 08 July 2011
Published: 25 October 2011
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