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| The Effects of MGF-Ct24E on the Proliferation and Gene Expression of Primary Osteoblasts |
| XIN Juan1,2, LIN Fu-chun1,2, ZHANG Bing-bing1,2, XIANG Yan1,2, WANG Yuan-liang1,2 |
1. Key Laboratory of Biorheological Science and Technology Chongqing University, Ministry of Education, Chongqing 400030, China;
2. Research Center of Bioinspired Materials Science and Engineering of National "985 Project program" of China, Bioengineering College, Chongqing University, Chongqing 400030, China |
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Abstract To study the effect of the last 24 amino acids peptide(MGF-Ct24E) corredsponding to the C-terminal part of the mechano growth factor(MGF) E domain on bone formation in vitro, primary osteoblasts were obtained from neonatal rat. MTT assay and cell cycle assay were used to study the proliferation and cell cycle stage distribution of osteoblasts. The gene expression profile of osteoblasts after treat with MGF-Ct24E was measured by DNA microarray analysis. Quantitative PCR was used to verify the microarray data. The results suggested that the proliferation activity of MGF-Ct24E group was significantly higher at the first day compared with control. The significantly greater proportion of S and G2/M phase cells appeared in the MGF-Ct24E group than control. Microarray data showed that 1397 genes were identified to be differentially expressed in the MGF-Ct24E group. Among these identified genes,922 genes were up-regulated and 475 genes were down-regulated. Most of the differentially expressed genes were associated with the regulation of proliferation and differentiation, growth factor activity and binding. The effect of MGF-Ct24E on the regulation of cell proliferation and differentiation suggest a potential role in the treatment of bone repair.
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Received: 14 April 2011
Published: 25 October 2011
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