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Preparation and Identification of the Specific Antibody against Diethylstilbestrol
DENG Sheng-liang, LI Ping, LIU Hong-bin, CHENG Ma-li, YU Hong-xia, CHEN Gang, YANG Shu-ming
China Biotechnology ›› 2011, Vol. 31 ›› Issue (06) : 106-110.
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Preparation and Identification of the Specific Antibody against Diethylstilbestrol
Diethylstilbestrol was activated by ethyl 4-bromobutyrate to introduce carboxyl group and then conjugated to BSA via EDC/NHS as immunogen, New Zealand rabbits were immunized and antisera were prepared. The results showed that complete antigen was successfully synthesized and specific antibody was generated, with titer of 1.28×105 the antisera cross-reactivity with hexestrol (9.34%), dienestrol (1.1%) and no cross-reactivity with 17β-estradiol. An indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed for determination of DES. In the linear range between 1 and 30 ng/ml, IC50 of 7 ng/ml and a detection limit of 0.3 ng/ml for DES were reached. Therefore the assay as screening method can meet the demand of DES residue detection for animal food products.
Diethylstilbestrol / Specific antibody / ELISA {{custom_keyword}} /
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