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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (03): 97-100    DOI:
    
The Application of Reverse Genetics to the Live-attenuated RSV Vaccine
FU Yuan-hui1, HE Jin-sheng1, HONG Tao1,2
1. College of Life Sciences & Bioengineering, Beijing Jiaotong University, Beijing 100044, China;
2. Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
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Abstract  

Human respiratory syncytial virus (RSV) is the leading viral agent of serious respiratory disease in infants and young children worldwide, infecting nearly all children one or more times by age 2. An intranasal live-attenuated RSV vaccine mimics natural infection and presumably induces the same broad array of local and systemic immunity, including innate and cell-mediated immunity as well as mucosal and serum antibodies. The intranasal route is albe to escapes the immunosuppressive effects of maternal serum antibodies. Additionally, the immune-mediated enhanced disease, induced by nonreplicating killed RSV vaccine, is not associated with attennated RSV vaccine candidates. A number of live-attenuated RSV vaccine candidates have been developed by conventional methods. However, there are many advantages for developing attenuated strains by reverse genetics.The application of reverse genetics to the live-attenuated RSV vaccine is reviewed.



Key wordsRespiratory syncytial virus      Reverse genetics      Live-attenuated vaccine     
Received: 30 September 2010      Published: 01 April 2011
ZTFLH:  Q939.44  
Cite this article:

FU Yuan-hui, HE Jin-sheng, HONG Tao. The Application of Reverse Genetics to the Live-attenuated RSV Vaccine. China Biotechnology, 2011, 31(03): 97-100.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I03/97

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