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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (03): 91-96    DOI:
    
The Development and Recent Improvements of in Vitro Nucleic Acid Amplification Technology
LV Bei1, CHENG Hai-rong2, YAN Qing-feng4, HUANG Zhen-ju3, LI Yi-nv1, LUO Da5, SHEN Gui-fang1, ZHANG Zhi-fang1, DENG Zi-xin2, LIN Min1, CHENG Qi1
1. Biotechnology Research Institure,Chinese Academy of Agricultural Sciences,Beijing 100081, China;
2. Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;
3. Zhejiang Taijing Biotechnology Limited, Ningbo 315100, China;
4. College of Life Science,Zhejiang University, Hangzhou 310058, China;
5. College of Life Science, Sun Yat-sen University, Guangzhou 510275, China
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Abstract  

Rapid nucleic acid amplification in vitro is a revolutionary technology developed since 1983. It has been applied widely in modern agriculture, medical and food science etc.. Such technology has provided a great tool for gene manipulation and functional genomics. In vitro nucleic acid amplification is usually carried out by a thermal stable enzyme, such as Taq polymerase, and requires thermal cycle equipment. The invention of isothermal amplification has improved the traditional PCR method and in some way simplified the reaction procedure. Recombinase-aid amplification (RAA) was introduced. RAA is the latest isothermal amplification technology which may be carried out under the room temperatures. The distinctive merit of RAA is mimicking in vivo T4-phage DNA amplification complex and the whole reaction is super fast and specific. Moreover, it can also be used for digital real time quantitative analysis.



Key wordsRecombinase-aid      Nucleic acid amplification in vitro      Isothermal     
Received: 11 November 2010      Published: 01 April 2011
ZTFLH:  Q784  
Cite this article:

LV Bei, CHENG Hai-rong, YAN Qing-feng, HUANG Zhen-ju, LI Yi-nv, LUO Da, SHEN Gui-fang, ZHANG Zhi-fang, DENG Zi-xin, LIN Min, CHENG Qi. The Development and Recent Improvements of in Vitro Nucleic Acid Amplification Technology. China Biotechnology, 2011, 31(03): 91-96.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I03/91

[1] Gill P, Ghaemi A, Nucleic acid isothermal amplification technologies: a review. Nucleosides, nucleotides & nucleic acids, 2008, 27:224-243.
[2] Guatelli J C, Whitfield K M, Kwoh D Y, et al. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of the National Academy of Sciences of the United States of America, 1990, 87:1874-1878.
[3] Compton J, Nucleic acid sequence-based amplification. Nature, 1991, 350: 91¨C92.
[4] Walker G T, Little M C, Nadeau J G, et al. Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Proceedings of the National Academy of Sciences of the United States of America, 1992a, 89:392-396.
[5] Walker G T, Fraiser M S, Schram J L, et al. Strand displacement amplification--an isothermal, in vitro DNA amplification technique. Nucleic acids research, 1992b, 20, 1691-1696.
[6] Vincent M, Xu Y, Kong H, Helicase-dependent isothermal DNA amplification. EMBO reports, 2004, 5:795-800.
[7] Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res, 2000, 28:E63.
[8] Lizardi P M, Huang X, Zhu Z, et al. Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nature Genet, 1998, 19:225-232.
[9] Piepenburg O, Williams C H, Stemple D L,et al. DNA detection using recombination proteins. PLoS Biol, 2006, 4:e204.
[10] Thekisoe O M, Bazie R S, Coronel-Servian A M, et al. Stability of loop-mediated isothermal amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates. The Journal of veterinary medical science, 2009, 71:471-475.
[11] Lee D, La Mura M, Allnutt T R, et al. Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences. BMC biotechnology, 2009, 9:7.
[12] Yamamura M, Makimura K, Ota Y. Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis. Japanese journal of infectious diseases, 2009, 62:20-25.
[13] Yamazaki W, Taguchi M, Kawai T, et al. Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples. Applied and environmental microbiology, 2009, 75:1597-1603.
[14] Yoshino M, Watari H, Kojima T, et al. Rapid, sensitive and simple detection method for koi herpesvirus using loop-mediated isothermal amplification. Microbiology and immunology, 2009, 53:375-383.
[15] Zhang H, Om M T, Aboge G O, et al. Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method. Experimental parasitology, 2009, 122(1):47-50.
[16] Kono T, Savan R, Sakai M, et al. Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. Journal of virological methods, 2004, 115:59-65.
[17] Thekisoe O M, Inoue N, Kuboki N, et al. Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs. Veterinary parasitology, 2005, 130:327-330.
[18] Kurosaki Y, Takada A, Ebihara H, et al. Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification. Journal of virological methods, 2007, 141:78-83.
[19] Kaneko H, Kawana T, Fukushima E, et al. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. Journal of biochemical and biophysical methods, 2007, 70:499-501.
[20] Aryan E, Makvandi M, Farajzadeh A, et al. A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex. Microbiological Research, 2010, 165:211-220.
[21] 彭涛. 核酸等温扩增技术及其应用. 北京:科学出版社,2009, 98. Peng T. The Application of Isothermal Nucleic Acid Amplification Technologies. Beijing: Science Press, 2009, 98.
[22] Benkovic S J,Salinas F. Methods for nucleic acid manipulation, US Patent, 6929915,2005.
[23] Piepenburg O, Williams C H, Armes N A. Recombinase polymerase amplification, US,2005/0112631,2005.
[24] Hardy C D, Martin P K. Isothermal SNP detection method, US,2008/0118917,2008.
[25] 吕蓓,程海荣,严庆丰,等. 用重组酶介导扩增技术快速扩增核酸. 中国科学:生命科学,2010,40 (10):983-988. Lü B, Cheng H R, Yan Q F, et al.Scientia Sinica Vitae, 2010, 40(10):983-988.

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[2] SHI Zhong-lin,CUI Jun-sheng,YANG Ke,HU An-zhong,LI Ya-nan,LIU Yong,DNEG Guo-qing,ZHU Can-can,ZHU Ling. Research Progress in Isothermal Amplification of Nucleic Acid Based on Microfluidic Chip[J]. China Biotechnology, 2021, 41(2/3): 116-128.
[3] Jing WANG,Xin XU,Xue-yu WANG,Lun-guang YAO,Yun-chao KAN,Jun JI. Research Progress of Loop-Mediated Isothermal Amplification in Food Safety Testing[J]. China Biotechnology, 2018, 38(11): 84-91.
[4] DENG Han-chao, YIN Chang-cheng, LIU Guo-zhen, LIN Jian-rong, DENG Ping-jian. Progress in Nucleic Acid Detection Techniques for Genetically Modified Organisms[J]. China Biotechnology, 2011, 31(01): 86-95.
[5] XU Xi-Gang, LI Su-Long, YANG Jun-Hong, LI Dan-Dan, JIANG Yan-Chun, XIE Xiao-Feng, CUI Li-Chun. Establishment of Rapid Detection Method for Vibrio vulnificus in Aquatic Food by Loop-Mediated Isothermal Amplification[J]. China Biotechnology, 2010, 30(06): 96-102.
[6] GAO Shan-Dian, CHANG Hui-Yun, DU Jun-Zheng, CONG Guo-Zheng, SHAO Jun-Jun, LIN Tong. Transcription Mediated Amplification and Its Applications in Diagnosis[J]. China Biotechnology, 2010, 30(04): 120-124.
[7] . Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis[J]. China Biotechnology, 2009, 29(01): 80-85.
[8] . Development of Loop-Mediated Isothermal Amplification ( LAMP ) Method for Detection of Vibrio parahaemolyticus[J]. China Biotechnology, 2007, 27(12): 66-72.