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| Isolation and Purification of Bromoperoxidase from Corallina Officinalis Using Ultrafiltration Membrane |
| CHENG Xiao-fei1, ZHANG Biao-ming2,3, XUE Song2, XIAO Tong-hu1 |
1. Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China;
2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is developed, which is consist of two steps: crude enzyme extraction was heated at 70℃ for 2h, and then was ultrafiltered with PVDF membrane. The ultrafiltration conditions were optimized, and the optimized conditions are: MWCO=100kDa, 0.02MPa pressure, 600r/min stirring, 0.12mg/ml initial protein concentration and pH=6.0. The crude extraction purified with heat treatment and then ultrafitration results a 21-fold purification, with a 96% overall yield and 212U/mg specific activity.
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Received: 19 November 2010
Published: 01 April 2011
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Corresponding Authors:
CHENG Xiaofei
E-mail: xthci@sina.com
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