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中国生物工程杂志

China Biotechnology
China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (02): 91-94    DOI:
    
Rapid Measurement of Growth of Bacteria Grown in the Precipitate-containing Liquid Medium
ZHAO Gen-gui1,2, XUE Xiao-chun2, YANG Su-ping1
1. Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China;
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China
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Abstract  

Objective: To eliminate the interference of precipitate caused by inorganic salt in liquid medium on bacterial growth determination by turbidimetry. Methods: Using Methylobacterium organophilium ME25 as a model system, the effects of chelator EDTA on optical density determination and precipitate removal with spectrophotometer are investigated. Results: Chelator EDTA of 1.25×10-2mol/L could eliminate completely the precipitate in medium within a minute in the pH from 4.0 to 11.0 at room temperature, and the stability of sample is sustained more than an hour and didn’t affect the bacterial density determination at 600 nm (OD660). The data of optical density measured with real samples and standard samples showed the relative error of 3.0%, recovery of 98.0%~100%, relative standard deviation (RSD) of 0.5%. Conclusion: Chelator EDTA was quickly able to remove inorganic salt precipitate in medium and eliminating the interference of precipitate on bacterial growth determination.A relatively reliable, accurate and precise method used to measure bacterial optical density in precipitate-containing liquid medium was proposed.

Key words： EDTA      Precipitate      Biomass determination      Methylobacterium organophilium     
Received: 01 November 2010      Published: 18 February 2011
ZTFLH:  Q819  
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DIAO Hen-Gui
XUE Xiao-Chun
YANG Su-Ping
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ZHAO Gen-gui, XUE Xiao-chun, YANG Su-ping. Rapid Measurement of Growth of Bacteria Grown in the Precipitate-containing Liquid Medium. China Biotechnology, 2011, 31(02): 91-94.

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https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/91

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