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中国生物工程杂志

China Biotechnology
China Biotechnology  2011, Vol. 31 Issue (02): 85-90    DOI:
    
The Selection of Defoamer for the Antimicrobial Lipopeptide Fermentation by Bacillus amyloliquefaciens ES-2 and Extraction and Purification of Lipopeptide
WEI Hao, LU Zhao-xin, LV Feng-xia, ZHAI Ya-nan, GUO Hao, HAO Hui, BIE Xiao-mei
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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Abstract  

Three kinds of defoamers—soybean oil, organic silicone and polyether were used for the antimicrobial lipopeptide fermentation by Bacillus amyloliquefaciens ES-2, and the results showed that soybean oil not only function as a good defoamers, but could also enhance lipopeptide production and got a high yield of 2497.67mg/L. After extraction, the purity of the antimicrobial lipopeptide is 55.68%, and the extraction rate reached 88.72%. The effect of adsorption and desorption of different macroporous resin were also studied, and the result indicated that X-5 macroporous resin was the most suitable for the purification of antibacterial lipopeptide. The optimized technological parameters of the purification were as following: loading concentration 14.4mg/ml, flow rate for adsorption 1ml/min,desorption flow rate 2 ml/min, and elution volume was 2.5 BV. With above conditions, the recovery ratio and purity of product reached 90.01%and 74.4% separately.



Key wordsAntimicrobial lipopeptide      Defoamer      Macroreticular resin     
Received: 13 October 2010      Published: 18 February 2011
ZTFLH:  Q819  
Cite this article:

WEI Hao, LU Zhao-xin, LV Feng-xia, ZHAI Ya-nan, GUO Hao, HAO Hui, BIE Xiao-mei. The Selection of Defoamer for the Antimicrobial Lipopeptide Fermentation by Bacillus amyloliquefaciens ES-2 and Extraction and Purification of Lipopeptide. China Biotechnology, 2011, 31(02): 85-90.

URL:

https://manu60.magtech.com.cn/biotech/     OR     https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/85

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