On the Development of a Microsphere-based Multiplexed Immunoassay for Cephalexin and Ractopamine in Raw Milk

China Biotechnology

2011, Vol. 31

Issue (02): 79-84 DOI:

On the Development of a Microsphere-based Multiplexed Immunoassay for Cephalexin and Ractopamine in Raw Milk

LIU Tian-long^1,2, WANG Yan-fei¹, ZOU Ming-qiang¹, LIU Xiao-lei², YUN Cai-lin²

1. Chinese Academy of Inspection and Quarantine, Beijing 100123, China;
2. Inner Mongolia Medical College, Hohhot 010059, China

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Abstract

A rapid and convenient multiplexed, microsphere-based immunoassay was simultaneously developed for Cephalexin(Cex) and Ractopamine(Rac) in raw milk. Synthetic antigen of Cephalexin and Ractopamine were covalently coupled to fluorescent-coded polystyrene microspheres as capture antigen, which were used to build a competitive detection system with standard sample of antibiotics and monoclonal antibody against Cephalexin and Ractopamine. Phycoerythrin-labeled goat anti mice IgG as fluorescent probe labeled monoclonal antibody specifically react with capture antigen. Fluorescent intensity associating microsphere and probe were detected respectively by laser on suspension array system to complete final detection. No cross-reactivity of the antibody was observed in reaction system, indicating that the antibody is highly specific for Cephalexin and Ractopamine. Optimum concentration of antigen covered microsphere was set up (8.4μg and 16.64μg per 100μl microspheres). Finally, the performance of beads-based immunoassay for simultaneous determination of Cephalexin and Ractopamine were evaluated demonstrating that the beads-based immunoassay is more adjective method for Cephalexin and Ractopamine determination in raw milk (LOD Cex = 20.59 ng/ml;LOD Rac = 23.51 ng/ml).

Key words： Residues of antibiotics and food additives Immunoarray Suspension Array System

Received: 25 October 2010 Published: 18 February 2011

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LIU Tian-long, WANG Yan-fei, ZOU Ming-qiang, LIU Xiao-lei, YUN Cai-lin. On the Development of a Microsphere-based Multiplexed Immunoassay for Cephalexin and Ractopamine in Raw Milk. China Biotechnology, 2011, 31(02): 79-84.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/79

[1] 江岚. 瘦肉精中毒抢救24 例分析. 中国现代医生,2009, 47(30):130-131. Jiang L. Chinese Modern Doctor,2009, 47(30):130-131.
[2] 周少峰. 一起因食用猪肝引起瘦肉精中毒的调查分析.实用预防医学, 2002, 9( 6 ): 706. Zhou S F.Preventive Medicine, 2002, 9( 6 ): 706.
[3] Martine Z, Navaari J E. Food poisoning related to consumption of illcit B-agonist in liver. Lancet, 1990, 336 (8726):1311-1315.
[4] 王怀良,王永利.方理本等.药理学.北京:人民卫生出版社,2001.45-46. Wang H L,Wang Y L, Fang L B, et al.Pharmacology.Beijing:The People’s Medical Publishing House,2001.45-46.
[5] 李华.抗生素使用存在的问题及分析. 兽医卫生,2009,9:38-41. Li H. Veterinary Hygiene,2009,9:38-41.
[6] Monica L. Miquel P. Montserrat G, et al. Physicochemical properties of enrofloxacin. Journal of Pharmaceutical and Biomedical Analysis,1997,15:1845-1849.
[7] 张琦, 叶能胜. 固相萃取2胶束电动色谱法测定牛奶中的4种β-内酰胺类抗生素. 色谱,2008,26(6):682-686. Zhang Q, Ye N S. Chromatogram,2008,26(6):682-686.
[8] 蔡玉娥,蔡亚岐. 高效液相色谱2紫外光度法检测尿液和牛奶中多种头孢类抗生素. 分析化学,2006, 34 (6) : 745-748. Cai Y E, Cai Y Q.Chinese Journal of Analytical Chemistry,2006, 34 (6) : 745-748.
[9] 黄百芬,吴丹青. 超高效液相色谱-串联质谱法同时测定牛奶中19种β-内酰胺类抗生素. 中国卫生检验杂志,2010,20(1):1-6. Huang B F, Wu D Q.Chinese Journal of Health Laboratory Technology,2010,20(1):1-6.
[10] 涂晓真,杨志杰,等. 反相高效液相色谱对多种头孢菌素的分离和测定. 中国药师,2003,6 (10):635-637. Tu X Z, Yang Z J.China Pharmacist,2003,6 (10):635-637.
[11] 谢会玲,陈伟. 动物源食品中β-内酰胺类抗生素多残留免疫分析方法研究进展. 食品科学,2008,29(7):490-494. Xie H L,Chen W.Bromatology ,2008,29(7):490-494.

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