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| Cloning, Expression and Biological Activity Study of Human Peptide Tyrosine Tyrosine and Its Derivative |
| WANG Xiao-xi, LI Juan, LAO Xun, ZHANG Hong-dan, HUANG Jing, WU Zi-rong |
| School of Life Science, East China Normal University, Shanghai 200062, China |
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Abstract Peptide tyrosine tyrosine (PYY) is a 36 amino-acid peptide secreted from L-cells predominantly within the distal gastrointestinal tract. Two main endogenous forms of PYY have been identified, PYY1-36 and PYY3-36. The latter has an effect of anorexia and can inhibit food intake in rodent animals. Peripheral administration of PYY3-36 can reduce food intake and body weight gain. All PYY3-36 reported were chemically synthesized. Gene cloning of PYY3-36 will be an innovation. PYY3-36 gene and its derivative that were obtained by site-directed mutagenesis and artificial synthesis respectively were cloned into pET32a(+) vector. Recombinant plasmids were expressed in Escherichia coli. Fusion proteins were purified by Ni ion affinity chromatography. Purified fusion proteins were then digested by enterokinase and purified by another affinity chromatography to get the target peptides. The biological activities of PYY3-36 and PYY3-36-Gly37 were studied in vivo in Kunming mice. The result showed that when administrated at a dose of 800μg/kg intraperitoneally, both PYY3-36 and PYY3-36-Gly37 can reduce food intake within 9 hours. Interestingly, the inhibition ratio of PYY3-36-Gly37 can reach to 50%, which is obviously higher than that of PYY3-36.
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Received: 13 October 2010
Published: 18 February 2011
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