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| Nitric Oxide Signaling in Chitooligosaccharide Induced Tobacco Defense Response |
| ZHANG Hong-yan1,2, WANG Wen-xia1, YIN Heng1, LU Hang1,2, ZHAO Xiao-ming1, DU Yu-guang1 |
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian 116023, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract Chitooligosaccharide (COS) or oligochitosan has been found to induce plant defense response. In order to study the mechanism of COS induced tobacco defense response, nitric oxide (NO) generation was indentified in tobacco suspension cells induced by COS by using NO specific fluorescence diaminofluorescein diacetate (DAF-2DA) and Microplate Reader GEMINI EM. The results showed that tobacco cells treated with COS resulted in a rapid NO generation, whose fluorescence intensity was dependent of COS concentration. The NO generation was sensitive to NO scavenger Carboxy-PTIO potassium salt (cPTIO), NO synthase (NOS) inhibitor Nω-nitro-L-arginine methyl Ester (L-NAME), lanthanum chloride and ruthenium red. Nitrate reductase (NR) inhibitors sodium azide and sodium tungstate had no effect on NO generation induced by COS. L-NAME, cPTIO, EGTA, lanthanum chloride and ruthenium red reduced the expression of defense-related gene induced by COS. Above results showed that NOS mediated NO generation and Ca2+ participated in the process of COS-induced NO generation.
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Received: 30 September 2010
Published: 18 February 2011
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