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| In vivo Bioluminescence Imaging of Luc Labeled Human PSCA-expressed Tumor in Mouse Model |
| DONG Lei1,2, ZHANG Xiao-peng1, YI Shao-qiong1, MAO Ya-li3, YU Ting1, HOU Li-hua1, FU Ling1, YU Chang-ming1, CHEN Wei1 |
1. Department of Molecular Biology, State Key Laboratory of Pathogens and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China;
2. Clinical Laboratory Center, The General Hospital of PLA Air Force, Beijing 100142, China;
3. Department of Pharmacology, The General Hospital of PLA Air Force, Beijing 100142, China |
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Abstract Objectives:To establish a tumor model in mouse with expressing Luc and human PSCA for the development of new anti-tumor drugs or vaccines. Methods:The Luc and human PSCA genes were cloned and pcDNA-luc and pcDNA-PSCA plasmids were constructed. RM-1 cells were stably transfected with both pcDNA-luc and pcDNA-PSCA plasmids. The Luc-expressed cells were examined with luminometer and the PSCA-expressed cells were examined with RT-PCR and flow cytometry analysis. The male C57BL/6 mice were inoculated with RM-PSCA/Luc cells, then the growth and the survival time of mice were measured, respectively. In vivo bioluminescence imaging was used to detect Luc expression and immunohistochemistry analysis was used to detect PSCA expression. Results:RM-PSCA/Luc cells stably expressing Luc and PSCA were obtained and can establish tumor growth in 100% of inoculated mice. Conclusion:The Luc-expressed and PSCA-expressed tumor model in mice was successfully established and could be used for evaluating the activities of drugs or vaccines.
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Received: 01 November 2010
Published: 18 February 2011
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Cite this article:
DONG Lei, ZHANG Xiao-peng, YI Shao-qiong, MAO Ya-li, YU Ting, HOU Li-hua, FU Ling, YU Chang-ming, CHEN Wei. In vivo Bioluminescence Imaging of Luc Labeled Human PSCA-expressed Tumor in Mouse Model. China Biotechnology, 2011, 31(02): 1-6.
URL:
https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I02/1
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