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| Detection of Allergen Mustard Components in Food by Real-time Fluorescent PCR |
| SHAO Juan1, CAO Ji-juan2, LIU Yang2, WANG Chang-wen2, ZHAO Tong-tong2, LI Jing-quan3 |
1. School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China;
2. Liaoning Entry-Exit Inspection & Quarantine Bureau, Dalian 16001, China;
3. TaKaRa Biotechnology(Dalian CO., LTD., Dalian 116600, China |
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Abstract Rapid identification of mustard materials in plant raw material products and plant-based foods is essential for effective control of a potential source of Allergen pathogens. A convenient Real-Time fluorescence polymerase chain reaction (PCR)-based assay which allowed detection and identification of a mustard -specific Housekeeping gene sin AI DNA sequence in foodstuffs and food was developed. The experiment results show that: the primers and probes could specific identify three kinds of mustard with 21 kinds of samples. Sensitivities results show that: 1 mg/kg for mustard of foods could be detected. Moreover, the four commercial samples (including mustard seeds) and deep processing of mustard allergen reference material (glucose) were detected and the detection results show that mustard allergen components were detected well.
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Received: 10 September 2010
Published: 25 January 2011
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