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| Effects of Tumor Necrosis Factor-alpha on the Osteogenic Differentiation of Mouse Bone Marrow-derived Mesenchymal Stem Cells |
| HE Lin-lin1,2, LAN Fei2, XUE Xiao-ping1 |
1. Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China;
2. Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, USA 30912 |
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Abstract Objective: To investigate the effects of tumor necrosis factor-alpha (TNF-α) on the osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells(BM-MSCs). Methods: BM-MSCs were cultured in osteogenic media (OS) with TNF-α using BM-MSCs in OS without TNF-α as a control group. Alkaline phosphatase staining was tested on the 7 days and Alizarin Red S (ARS) staining and quantification were tested on the 21 days. On the 7 days, mRNA were extracted from one set of the cells and Runx2 and Osterix mRNA expression were analysed by real-time RT-PCR , another set of the cells were lysed and Runx2 and Osterix protein expression were analysed by SDS-PAGE. Results: TNF-α inhibits alkaline phosphatase activity. TNF-α inhibits mineralized nodules formation. TNF-α inhibits Runx2 and Osterix mRNA and protein expression. Conclusion: TNF-α inhibition of the osteogenic differentiation of mouse BM-MSCs, in part, is through suppressing Runx2 and Osterix mRNA and protein expression which are the two key transcription factors of osteogenic differentiation.
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Received: 11 August 2010
Published: 25 January 2011
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