The Study of the Interaction Between Hepcidin and Fpn by FRET

China Biotechnology

2011, Vol. 31

Issue (01): 56-60 DOI:

The Study of the Interaction Between Hepcidin and Fpn by FRET

LIU Xiao-zhi^1,2, ZHAO Hui-jing³, CHEN Wei-bin^1,2, CHANG Yan-zhong¹, DUAN Xiang-lin¹

1. The Institute of Molecular Neurobiology and Neurpharmacology, Hebei Normal University, Shijiazhuang 050016, China;
2. North China Pharmaceutical Group, New Drug Research and Development Center, Shijazhuang 050050, China;
3. The NO.41 Middle School of Shijiazhuang City, Shijazhuang 050081, China

Download:

HTML

PDF(495KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

Iron is an essential trace element of life, ferroportin (Fpn) is the intestinal absorption of iron release of important cell proteins. Newly discovered antimicrobial peptide hepcidin secreted by the liver can regulate the important role of intestinal iron absorption, but still play a role in the lack of Fpn and experimental basis for hepcidin. So fluorescence resonance energy transfer technology (fluorescence resonance energy transfer, FRET) on the role of hepcidin and Fpn relationship between the depth study. First of all, were hepcidin-CFP fusion protein expression vector and identification; then with YFP, Fpn-YFP gene in animal cell expression vector, expression, and FRET detection. The results confirmed that hepcidin and Fpn direct interaction between, and found that two proteins interact in the cytoplasm after the distribution of hepcidin also. The results for the treatment of disorders of iron metabolism to provide a new and important theoretical basis for therapeutic strategies.

Key words： Fluorescence resonance energy transfer(FRET) Hepcidin Ferroportin(Fpn)

Received: 12 August 2010 Published: 25 January 2011

ZTFLH:

Q78

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LIU Xiao-zhi
	ZHAO Hui-jing
	CHEN Wei-bin
	CHANG Yan-zhong
	DUAN Xiang-lin

Cite this article:

LIU Xiao-zhi, ZHAO Hui-jing, CHEN Wei-bin, CHANG Yan-zhong, DUAN Xiang-lin. The Study of the Interaction Between Hepcidin and Fpn by FRET. China Biotechnology, 2011, 31(01): 56-60.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2011/V31/I01/56

[1] Durand P Y.Iron therapy: is it different with peritoneal dialysis compared to haemodialysis?. Nephrol Ther, 2006, 2 Suppl 5: 341-346.

[2] Wallace D F, Dixon J L, Ramm G A, et al. A novel mutation in ferroportin implicated in iron overload. Journal of Hepatology, 2007, 46(5): 921-926.

[3] Atanasiu V, Manolescu B, Stoian I. Hepcidin--central regulator of iron metabolism. Eur J Haematol, 2007, 78(1): 1-10.

[4] Chaudhuri S, Banerjee A, Basu K, et al. Interaction of flavonoids with red blood cell membrane lipids and proteins: Antioxidant and antihemolytic effects. International Journal of Biological Macromolecules, 2007, 41(1): 42-48.

[5] Ji D, Lv W, Huang Z, et al. Fluorescence resonance energy transfer imaging of CFP/YFP labeled NDH in cyanobacterium cell.Journal of Luminescence, 2007, 122-123(2007): 463-466.

[6] Chirio-lebrun M C, Prats M. Fluorescence resonance energy transfer (FRET): theory and experiments. Biochemical Education, 1998, 26(4): 320-323.

[7] Slimane T A, Fontanges P, Trugnan G. GFP, FRAP, FLIP, FRET, PRIM, FLASH ect.New microscopic techniques using new fluorescent probes. An overview. Biology of the Cell, 1999, 91(3): 227-228.

[8] Munishkina L A, Fink A L. Fluorescence as a method to reveal structures and membrane-interactions of amyloidogenic proteins. Biochimica Biophys Acta,2007,1768(8):1862-1885.

[9] Theodorsson E. Haemochromatosis, hepcidin and disorders of iron metabolism: fields of substantial clinical relevance and current advances.. Scand J Clin Lab Invest, 2006, 66(2): 79-82.

[10] Chen Y, O’Donoghue M B, Huang Y F, et al. A surface energy transfer nanoruler for measuring binding site distances on live cell surfaces. Journal of American Chemical Society,2010,132(46):16559-16570.

No related articles found!

Viewed

Full text

Abstract

Cited

Shared

Discussed