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Overproduction of Glutamic Acid in Corynebacterium glutamicum with H+-atp Inactivation |
ZHANG Bo, LI Tie-min, YANG Zhi-yong, HU Yong-fei, LI Yu |
School of Life Science, Liaoning University, Shenyang 110036, China |
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Abstract In order to confirm the application feasibility of overproduction of glutamic acid in C. glutamicum with H+-ATP inactivation in the construction of genetically modified bacteria. The sequence encoding H+-ATPase γ subunit in C.glutamicum ATCC13032 was partially deleted by crossover PCR, and the mutant, in which H+-atp gene was inactivated, was obtained by insertion inactivation. The glutamic acid production and growth rate of the mutant were tested. The results showed that the maximum production of glutamic acid of the mutant was 51.6 g/L in 100 g/L glucose culture medium, with 42.9 % increase in contrast to the wild parent strain, and that growth rate of the mutant in measurement of the growth curves was lower than wild parent strain. The results suggest that H+-atp gene inactivated in C.glutamicum increases glutamic acid production, and slightly reduces growth rate of the bacteria.
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Received: 02 August 2010
Published: 25 January 2011
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