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| Progress of Chromosome Walking by PCR Amplification Techniques |
| LI Fu-peng1, WU Bao-duo2, MA Chao-zhi1, FU Ting-dong1 |
1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China;
2. Oil Crops Research Institute Chinese Academy of Agricultural Sciences, Wuhan 430062, China |
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Abstract PCR-based chromosome walking is mainly applied to isolate flanking sequence of known sequence. It is convenient for gene cloning, obtaining regulatory elements and filling in gaps of whole genome sequencing. These methods can be classified into two sorts: dependent-ligation PCR and independent-ligation PCR. The technologies of PCR-based chromosome walking technologies in recent years and compares principles and procedures of these methods were outlined. The advantages and disadvantages of dependent-ligation PCR and independent-ligation PCR were summarized respectively, and their efficiency and usefulness were evaluated for investigator to select the appropriate method.
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Received: 01 September 2010
Published: 25 December 2010
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