The Pilot Study on T4 Lysozyme Fermentation by <em>Pichia pastoris</em>

China Biotechnology

2010, Vol. 30

Issue (12): 49-52 DOI:

The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris

WU Jia-xin¹, YU Zhi-qiang¹, WANG Hua-li¹, GE Xin-mei¹, QI Peng¹, ZHENG Ying-hua¹, LIU De-hu², SONG Min¹, ZHANG Guo-dong¹, CAO Qin¹

1. Institute of Veterinary Drug, China Animal Husbandry Industry Co.,Ltd,Beijing 100091, China;
2. Institute of Biotechnology Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China

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Abstract

T4 lysozyme pilot-scale fermentation was studied. T4 lysozyme was induced and expressed by Pichia pastoris in the 200L fermentor. The yield of target protein was 1.69g /L with 192U/mg enzyme activity in the broth. The enzyme protein was freeze-dried. The weight of enzyme powder was 211.1g. T4 lysozyme content was 68.9%, and activity was 182U/mg in enzyme powder. The total yield was 66%. The T4 lysozyme pilot-scale process line was established successfully.

Key words： T4 lysozyme Pilot-scale Pichia pastoris

Received: 18 August 2010 Published: 25 December 2010

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	WU Jia-xin
	YU Zhi-qiang
	WANG Hua-li
	GE Xin-mei
	QI Peng
	ZHENG Ying-hua
	LIU De-hu
	SONG Min
	ZHANG Guo-dong
	CAO Qin

Cite this article:

WU Jia-xin, YU Zhi-qiang, WANG Hua-li, GE Xin-mei, QI Peng, ZHENG Ying-hua, LIU De-hu, SONG Min, ZHANG Guo-dong, CAO Qin. The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris. China Biotechnology, 2010, 30(12): 49-52.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2010/V30/I12/49


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